Analysis of the Synergistic Effect of Antibiotics with Copper Oxide Nanoparticles Against Pseudomonas aeruginosa Isolated from Different Clinical Cases
Article Sidebar
Main Article Content
Abstract
Synergism between antibiotics and nanoparticles is one of the most important ways to combat antibiotic resistance. Copper nanoparticles were produced using a green, environmentally friendly method of mixing aqueous Ficus Sycomorus leaf extract with copper sulfate. The work aimed to study the synergistic effect of six antibiotics with copper oxide nanoparticles prepared in a green approach against bacteria Pseudomonas aeruginosa isolated from patient. A total of 110 specimens were collected from patients with burn, wound and urine infections. in Samarra General Hospital, Tikrit Teaching Hospital, Tikrit Military Hospital, and Baghdad Medical City of both genders from July to September 2023. A bacteriological examination was conducted to select bacterial pathogens, with a particular focus on Pseudomonas aeruginosa. From a total 110 cases, 50 (45.5%) gave positive cultures for Pseudomonas aeruginosa. CuO-NPs was characterized using different analytical techniques, Scanning Electron Microscope (SEM). showed that the CuO-NPs have a spherical shape, its average size is 29.2 nm. Energy-dispersive X-ray )EDX( results confirmed the presence of copper and oxygen in the composition. The synergistic effect of six antibiotics with CuO-NPs was studied. By evaluating the minimum inhibitory concentration of P. aeruginosa. The results show the highest inhibition against bacterial isolates was due to the synergistic effect of CuO-NPs with Impenem, Ciprofloxacin, Ceftazidime, Polymyxin B, Amikacin, and Cefepime respectively, especially on isolate (15) with zones of inhibition/mm were 19.33 ±0.33, 18.33 ±0.33, 16.33 ±0.33, 15.33 ±0.33, 14.67 ±0.33, and 12.7±0.58, respectively. The results indicated that the synergistic effect gave clear zones of inhibition against all isolates compared with the inhibition effect of nano-copper oxide and antibiotics.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
References
[1] Romi, Z. M., & Ahmed, M. E. (2024). The Synergistic Effect of Biosynthesized Copper Oxide Nanoparticles and Vancomycin on Biofilm Formation of Staphylococcus haemolyticus. Ibn AL-Haitham Journal For Pure and Applied Sciences, 37(2), 28-40. https://doi.org/10.30526/37.2.3374
[2] Theuretzbacher, U., Outterson, K., Engel, A., & Karlén, A. (2020). The global preclinical antibacterial pipeline. Nature Reviews Microbiology, 18(5), 275-285. https://doi.org/10.1038/s41579-019-0288-0
[3] Murray, C. J., Ikuta, K. S., Sharara, F., Swetschinski, L., Aguilar, G. R., Gray, A., ... & Tasak, N. (2022). Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. The lancet, 399(10325), 629-655. https://doi.org/10.1016/S0140-6736(21)02724-0
[4] Theuretzbacher, U. (2020). Dual-mechanism antibiotics. Nature Microbiology, 5(8), 984-985. https://doi.org/10.1038/s41564-020-0767-0
[5] León-Buitimea, A., Garza-Cárdenas, C. R., Garza-Cervantes, J. A., Lerma-Escalera, J. A., & Morones-Ramírez, J. R. (2020). The demand for new antibiotics: antimicrobial peptides, nanoparticles, and combinatorial therapies as future strategies in antibacterial agent design. Frontiers in microbiology, 11, 1669. https://doi.org/10.3389/fmicb.2020.01669
[6] Odeh, L. H., Talib, W. H., & Basheti, I. A. (2018). Synergistic effect of thymoquinone and melatonin against breast cancer implanted in mice. Journal of cancer research and therapeutics, 14(Suppl 2), S324-S330. 1669. https://doi.org/10.4103/0973-1482.235349
[7] Wen, W., Lowe, G., Roberts, C. M., Finlay, J., Han, E. S., Glackin, C. A., & Dellinger, T. H. (2018). Pterostilbene suppresses ovarian cancer growth via induction of apoptosis and blockade of cell cycle progression involving inhibition of the STAT3 pathway. International Journal of Molecular Sciences, 19(7), 1983. https://doi.org/10.3390/ijms19071983
[8] Tran, C. D., Makuvaza, J., Munson, E., & Bennett, B. (2017). Biocompatible copper oxide nanoparticle composites from cellulose and chitosan: facile synthesis, unique structure, and antimicrobial activity. ACS applied materials & interfaces, 9(49), 42503-42515. https://doi.org/10.1021/acsami.7b11969
[9] Zhou, J., You, Z., Xu, W., Su, Z., Qiu, Y., Gao, L., ... & Lan, L. (2019). Microwave irradiation directly excites semiconductor catalyst to produce electric current or electron-holes pairs. Scientific Reports, 9(1), 5470. https://doi.org/10.1038/s41598-019-41002-w
[10] Silva, N., Ramírez, S., Díaz, I., Garcia, A., & Hassan, N. (2019). Easy, quick, and reproducible sonochemical synthesis of CuO nanoparticles. Materials, 12(5), 804. https://doi.org/10.3390/ma12050804
[11] Hajnorouzi, A. (2020). Two ultrasonic applications for the synthesis of nanostructured copper oxide (II). Ultrasonics Sonochemistry, 64, 105020. https://doi.org/10.1016/j.ultsonch.2020.105020
[12] Qureshi, A., Blaisi, N. I., Abbas, A. A., Khan, N. A., & Rehman, S. (2021). Prospectus and development of microbes mediated synthesis of nanoparticles. Microbial nanotechnology: Green synthesis and applications, 1-15. https://doi.org/10.1007/978-981-16-1923-6_1
[13] Alam, M. S., Naseh, M. F., Ansari, J. R., Waziri, A., Javed, M. N., Ahmadi, A., ... & Garg, A. (2022). Synthesis approaches for higher yields of nanoparticles. In Nanomaterials in the Battle Against Pathogens and Disease Vectors (pp. 51-82). CRC Press. https://doi.org/10.1201/9781003126256-3
[14] Arya, A., Gupta, K., Chundawat, T. S., & Vaya, D. (2018). Biogenic synthesis of copper and silver nanoparticles using green alga Botryococcus braunii and its antimicrobial activity. Bioinorganic Chemistry and Applications, 2018(1), 7879403. https://doi.org/10.1155/2018/7879403
[15] Koçer, A. T., & Özçimen, D. (2023). A comprehensive study on extracellular green synthesis, antibacterial activity and process design of metallic nanoparticles from Botryococcus braunii microalga. JOM, 75(12), 5591-5605. https://doi.org/10.1007/s11837-023-05897-1
[16] Salem, W. M., Sayed, W. F., Hardy, M., & Hassan, N. M. (2014). Antibacterial activity of Calotropis procera and Ficus sycomorus extracts on some pathogenic microorganisms. African Journal of Biotechnology, 13(32). https://doi.org/10.5897/AJB2014.13981
[17] Salvo, J., & Sandoval, C. (2022). Role of copper nanoparticles in wound healing for chronic wounds: literature review. Burns & trauma, 10, tkab047. https://doi.org/10.1093/burnst/tkab047
[18] Linju, M. C., & Rekha, M. R. (2023). Role of inorganic ions in wound healing: an insight into the various approaches for localized delivery. Therapeutic Delivery, 14(10), 649-667. https://doi.org/10.4155/tde-2023-0036
[19] Elkobrosy, D., Al-Askar, A. A., El-Gendi, H., Su, Y., Nabil, R., Abdelkhalek, A., & Behiry, S. (2023). Nematocidal and bactericidal activities of green synthesized silver nanoparticles mediated by Ficus sycomorus leaf extract. Life, 13(5), 1083. https://doi.org/10.3390/life13051083
[20] Nzilu, D. M., Madivoli, E. S., Makhanu, D. S., Wanakai, S. I., Kiprono, G. K., & Kareru, P. G. (2023). Green synthesis of copper oxide nanoparticles and its efficiency in degradation of rifampicin antibiotic. Scientific Reports, 13(1), 14030. https://doi.org/10.1038/s41598-023-41119-z
[21] Alaallah, N. J., Abd Alkareem, E., Ghaidan, A., & Imran, N. A. (2023). Eco-friendly approach for silver nanoparticles synthesis
from lemon extract and their anti-oxidant, anti-bacterial, and anti-cancer activities. Journal of the Turkish Chemical Society Section A: Chemistry, 10(1), 205-216. https://doi.org/10.18596/jotcsa.1159851
[22] Adnan, W. G., & Mohammed, A. M. (2024). Green synthesis of chromium oxide nanoparticles for anticancer, antioxidant and antibacterial activities. Inorganic Chemistry Communications, 159, 111683 .https://doi.org/10.1016/j.inoche.2023.111683
[23] Velsankar, K., Sudhahar, S., Parvathy, G., & Kaliammal, R. (2020). Effect of cytotoxicity and aAntibacterial activity of biosynthesis of ZnO hexagonal shaped nanoparticles by Echinochloa frumentacea grains extract as a reducing agent. Materials Chemistry and Physics, 239, 121976.
[24] Thamer, N. A., Muftin, N. Q., & Al-Rubae, S. H. N. (2018). Optimization properties and characterization of green synthesis of copper oxide nanoparticles using aqueous extract of Cordia myxa L. leaves. Asian J. Chem, 30(7), 1559-1563. https://doi.org/10.14233/ajchem.2018.21242
[25] Taha, J. H., Abbas, N. K., & Al-Attraqchi, A. A. (2020). Green Synthesis and Evaluation of Copper Oxide Nanoparticles Using Fig Leaves and Their Antifungal and Antibacterial Activities. Int. J. Drug. Deliv. Technol, 10, 378-382. https://doi.org/10.25258/ijddt.10.3.13
[26] Bauer, A. W., Kirby, W. M. M., Sherris, J. C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology, 45(4_ts), 493-496.
https://doi.org/10.1093/ajcp/45.4_ts.493
[27] Eggleston, M., Park, S., & Parker, R. H. (1986). Clinical Pharmacology of Antibiotics: Review of Imipenem. Infection Control, 7(6), 333–338. doi:10.1017/S0195941700064377
[28] Abdul Wahab, A., Zahraldin, K., Ahmed, M. A. S., Jarir, S. A., Muneer, M., Mohamed, S. F., ... & Ibrahim, E. B. (2017). The emergence of multidrug-resistant Pseudomonas aeruginosa in cystic fibrosis patients on inhaled antibiotics. Lung India, 34(6),527-531.
https://doi.org/10.4103/lungindia.lungindia_39_17
[29] Shehabeldine, A. M., Amin, B. H., Hagras, F. A., Ramadan, A. A., Kamel, M. R., Ahmed, M. A., ... & Salem, S. S. (2023). Potential antimicrobial and antibiofilm properties of copper oxide nanoparticles: time-kill kinetic essay and ultrastructure of pathogenic bacterial cells. Applied Biochemistry and Biotechnology, 195(1), 467-485. https://doi.org/10.1007/s12010-022-04120-2
[30] Mohamed, A. H., & Kadium, S. W. (2022). Biological effect of copper oxide nanoparticles synthesized by Saccharomyces boulardii against of multidrug resistant bacteria isolated from diabetic foot infections. Cardiometry, (25), 31-40 https://doi.org/10.18137/cardiometry.2022.25.3140
[31] Dabos, L., Raczynska, J. E., Bogaerts, P., Zavala, A., Girlich, D., Bonnin, R. A., ... & Naas, T. (2023). Structural and biochemical features of OXA-517: a Carbapenem and expanded-spectrum Cephalosporin hydrolyzing OXA-48 variant. Antimicrobial agents and chemotherapy, 67(2), e01095-22.