Ethanol effect in induce histological lesions in Albino rat livers and using camel milk and olive oil to treat it
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Abstract
This study was designed to identify the influence of alcohol in the creation of histological lesions in the liver of Albino rats type Rattus norvegicus and treating these effects using camel milk and olive oil, in this study, 15 rats were randomly distributed into five groups each group containing three animals, the first group control group It was fed on a routine diet and administrated with distilled water, either groups 2, 3, 4,5 administrated with alcohol for 3 months at a dose began 10% V / V / day and gradually increase to up to 15% v / v / day, and then the animals Group 3 treated by ad libitum camel milk open dose for one month, Group 4 animals administrated with olive oil rate of 0.2 ml / kg body weight, Group 5 animals administrated by camel milk and olive oil together, the results of the current study, the second group showed the occurrence of a number of the histopathological lesions in the liver tissue consisted infiltration of lymphocytes with the occurrence of fibrosis within the liver tissue and thickening walls of the blood vessels that have suffered from the expansion of abnormal diameters ,in the third group most liver tissues return to semi-natural state had been observed after treating via camel milk for a one month with the presence of some simple histological changes in the liver tissue. The fourth group was also back to normal case in most of liver tissues. The fifth group was noted that the tissue response to the dual treatment was the best compared to the third and fourth groups and the tissue had completely healed and appeared as normal.
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References
1- Barrio, E.; Tome, S.; Rodriguez, I.; Gude, F.; Sanchez-Leira, J.; Perez-Becerra, E.;.(2014) Liver disease in heavy drinkers with and without alcohol withdrawal syndrome. Alcohol Clin Exp Res 28:131-136.
2- Mandayam, S; Jamal MM, and Morgan TR. (2004) Epidemiology of alcoholic liver disease. Semin Liver Dis;24:217-232.
3- Savolainen, V; Perola, M; Lalu, K; Penttila, A; Virtanen, I and Karhunen PJ.( 1995) Early perivenular fibrogenesis–precirrhotic lesions among moderate alcohol consumers and chronic alcoholics. J Hepatol;23:524-531.
4- Trabut, JB; Plat, A; Thepot, V; Fontaine, H; Vallet-Pichard, A; Nalpas, B;.( 2008) Influence of liver biopsy on abstinence in alcohol-dependent patients. Alcohol Alcohol;43:559-563.
6- Elnahas, A. (2008). Ultrasonographical examination of one humped Camels (Camelus dromedaries) liver withsome haematological and biochemical aspects. Ph. D. Thesis. Sudan University.
7- Farah, Z.; Rettenmaier, R. and Atkins, D.(2000).Vitamin content of Camel milk. J. Vit. Nutr. Res. 62: 30-33.
8- Shamsia, S. M. (2009). Nutritional and therapeutic properties of Camel and human milks. Inter. J. Gentic and Moleculer Biology. 1(2) pp: 52-58.
9- Yagil, R. (2000). Lactation in the desert Camel (Camelus romedarius ) In: Gahlot, T. K. (ed) selected Topics on Camel ids. The camel Publishers, Bikaner. India. pp: 61-72.
10- Konuspayeva, G.; Loiseau, G. and Faye, B. (2004). A better know led of Milk quality parameters: A preliminary step for improving the Camel milk market opportunity in a transition economythe case of Kazakhstan. Intl. Conf. 25-28.
11- Stark, AH. and Mader, Z:( 2002) Olive oil as a functional food: epidemiology and nutritional approaches. Nutr Rev, 60(6):170-176.
12- Lavelli V (2002) Comparison of the antioxidant activities of extra virgin olive oils. J Agric Food Chem, 50(26):7704-7708.
13- Moreau, R. and Lebrec, D.(2003) Acute renal failure in patients with cirrhosis: perspectives in the age of MELD. Hepatology;37: 233-243.
15- Laube, H.; Norris, H.T.; and Robbins, S.L.(2006) The nephromegaly of chronic alcoholics with liver disease Archives of Pathology 84:290–294,.
19- Bataller, R.; Schwabe, R.F.; Choi, Y.H.; Yang, L.; Paik, Y.H.; Lindquist, J.; Qian, T.; Schoonhoven, R.; Hagedorn, CH.; Lemasters, J.J. and Brenner, D.A.(2013) NADPH oxidase signal transduces angiotensin II in hepatic stellate cells and is critical in hepatic fibrosis . J Clin Invest; 112: 1383–1394.
20- Siqueira, C.; de Moura, MC.; Pedro, AJ.and Rocha P.(2008) Elevated nitric oxide and 3’,5’ cyclic guanosine monophosphate levels in patients with alcoholic cirrhosis. World J Gastroenterol; 14: 236-242.
21- Carmeliet, P.(2013) Angiogenesis in health and disease. Nat Med;9:653–660.
22- Garcia-Monzon, C.; Sanchez-Madrid, F.; Garcia-Buey, L.; Garcia-Arroyo, A.; Garcia-Sanchez, A. and Moreno-Otero, R. (2005) Vascular adhesion molecule expressionin viral chronic hepatitis: evidence of neoangiogenesis in portal tracts. Gastroenterology; 108:231–241.
23- Darwish ,H. A.; Abd Raboh, N. R. and Mahdy, A. (2012). Camels milk alleviates alcohol-induced liver injury in rats, food and chemical toxicology 50 Pp 1377-1380.
24- Nakbi, A.; Tayeb, W.; Grissa, A.; Issaoui, M.; Dabbou, S.; Chargui, I.; Ellouz, M.; Miled, A. and Hammami, M. (2010). Effects of Olive oil and its Fractions on Oxidative stress and the Livers Fatty Acid composition in 2,4-dichlorophenoxyacetic acid – treated rats, nutrition and metabolism 7:80
25- AL-Hashem, F. (2009). Camel's milk protects against Aluminum Chlorid induced toxicity in the liver and kidney of White Albino Rats .Ameri. J. of Biochemistry and Biotechnology.5(3): 98-109.
26- Abdel- Magjeed, N. A. (2005). Corrective effect of milk camel on some Cancer biomarkers in blood of Rat intoxicated with Aflaltoxin B1 .J. Saudi chem. Soc. 9: 253-264.
27- De La Cruz, JP. and Quintero, L. (2000) Auxiliadora Villalobos M, Sánchez de la Cuesta F: Lipid peroxidation and glutathione system in
hyperlipemic rabbits: influence of olive oil administration. Biochim Biophys Acta, 1485:36-44.
28- Bonanome, A.; Pagnan, A.; Biffanti, S.; Opportuno, A.; Sorgato, F.; Dorella, M.; Maiorino, M.and Ursini, F.(2012) Effect of dietary monounsaturated and polyunsaturated fatty acids on the susceptibility of plasma low density lipoproteins to oxidative modification. Arterioscler Thromb, 12:529-533.
29- Kyle, ME.; Miccadei, S.; Nakae, D. and Farber,
30- JL. (1997) Supeoxide dismutase and catalase protect cultured hepatocytes from the cytotoxicity of acetaminophen. Biochem Bioph Res Co, 149(3):889-896.
31- Ochoa-Herrera, JJ.; Huertas, JR.; Quiles, JL. and Mataix, J. (2001) Dietary oils high in oleic acid, but with different non-glyceride contents, have different effects on lipid profiles and peroxidation in rabbit hepatic mitochondria. J Nutr Biochem, 12(6):357-364.