Impact of the muscular Training on the levels of Sodium and Potassium and on the activity of lactate dehydrogenase in blood serum
Main Article Content
Abstract
The recent study aimed at evaluating the impact of the training intensity and the muscular stress the activity of (Na+ - K – ATP ase). The levels of the extra cellular (Sodium and Potassium) (blood serum) and the activity of the lactate dehydrogenase were evaluated for the handball players before and after exercising the sport training as well as evaluated the same data for the basketball players (The most stressed in training) before and after exercising these special game trainings. The research samples were gathered from (30) players of handball and basketball.
The blood serum isolated to perform the required tests by using the special kits of each test.
The results of measuring the extra cellular sodium showed a significant decrease (P≤ 0.05) for the handball players after exercising the sport training compared with the concentration before exercising the training. As well as the case of basketball players , the study results indicated that the sodium concentrations (P≤ 0.05) decreased significantly after exercising the sport training compared with the concentration before exercising the training. These results also showed the significant differences (P≤ 0.05) in comparison with the two player groups of handball and basketball before and after exercising the training.
As to the concentration of extra cellular potassium the results appeared a significant increase (P≤ 0.05) for the handball players after exercising the sport training compared with the concentration before exercising the training. As well as the case of basketball players , the results of study showed a significant increase at potassium concentration (P≤ 0.05) after exercising the sport training compared with the concentration before training. The results also appeared the absence of significant differences in comparison with the two – player groups before trainings. In comparison with potassium concentration after training between the two groups , the results gave significant different (P≤ 0.05) in which potassium was more concentrated in basketball group.
As to the lactate dehydrogenase activity , the results showed a significant increase in enzyme activity (P≤ 0.05) in the two – players groups after the training , but there were no significant differences in comparison with the both player groups of handball and basketball after performing the trainings.
This indicated to occurring changes in activity of (Na+ - K ATP ase) in case of happening the body stress , leading to decrease in concentration of extra cellular sodium and increase in concentration of extra cellular potassium and then followed some changes at level of these elements intra cellular. So this change increased with increasing the stress intensity.
Article Details

This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
References
1- Nordsborg, N. B.; Kusuhara, K.; Hellsten, Y.; Lyngby, S.; Lundby, C.; Madsen, K. and Pilegaard, K.(2010). Contraction-induced changes in skeletal muscle Na+,K+ pump mRNA expression – importance of exercise intensity and Ca2+-mediated signaling. Acta Physiologica. 198(4): 487–498.
3- Peter, L.; Jorgensen.; O, Kjell.; akansson, H.; Steven, J.D,. (2003). Structure andmechanism of na,k-atpase: Functional Sites and Their Interactions. Annu. Rev. Physiol. 65:817–49.
4- Zijian, Xie and Amir, Askari. (2002). Na+/K+-ATPase as a signal transducer. Eur. J. Biochem. 269, 2434–2439 .
5- Aslıhan, Aydemir ksoy.(2002). Na+ k+–atpase: a review. journal of ankara medical school .24 ( 2).
6- Clause, torben.(2003). Na_-K_ Pump Regulation and Skeletal Muscle Contractility. physiol rev . 83 (8).
7- Glitsch, HG.(2001). Electrophysiology of the Sodium-Potassium-ATPase in cardiac cells. physiological reviews. 81(4):1791-826.
9- Hillmann, G; Beyer, G;Z. Klin.(1967): Biochem.5 (93).
10- Trinder, P; (1951): Analyst 76: 596.
11- Amador, E.; Dorfaman, L. E.; Wacker, W. E. C.,(1963). Clin. Chem., 9:391 .
13- Clausen, T. (2003). The sodium pump keeps us going. Ann N Y Acad Sci. 986:595-602.
14- Hansen, AK. ;T, Clausen; OB., (2005). Nielsen. Effects of lactic acid and catecholamines on contractility in fast-twitch muscles exposed to hyperkalemia. Am J Physiol Cell Physiol. Jul; 289(1): C104-12. 15- Clausen, T.(2005). Na+-K+ pump stimulation improves contractility in damaged muscle fibers. Ann N Y Acad Sci. 1066:286-94.
17- Michael, J. McKenna.; Jens ,Bangsbo.; and Jean Marc, Renaud.(2008). Muscle K_, Na_, and Cl_ disturbances and Na_-K_ pump inactivation: implications for fatigue. JAppl Physiol 104: 288–295. 18- Elmashad, Wael Mohamed and Sarhan, Naglaa Ibrahim.(2014). Biochemical and Histological Study on the effect of muscular exercise on skeletal muscle of adult albino rats. Life Science Journal. 11(11).
19- Atanasovska, Tania; Aaron C., Petersen; David ,M. Rouffet, ; François, Billaut.; Irene, Ng.; Michael, J. McKenna.(2013). Plasma K+ dynamics and implications during and following intense rowing exercise. Articles in PresS. J Appl Physiol .May( 8).
20- Leppik, JA.; Aughey, RJ,; Medved, I.; Fairweather, I.; Carey, MF.; and McKenna, MJ. (2004).Prolonged exercise 430 to fatigue in humans impairs skeletal muscle Na+, K+-ATPase activity, sarcoplasmic reticulum Ca2+431 release and Ca2+ uptake. J Appl Physiol 97: 1414-1423.
21- Meludu, S.C; Nishimuta, M.; Yoshitake, Y.; Toyooka N.Kodama1 , C. S. Kim, F.; Maekawa, Y.; Fukuoka, H.(2002). Anaerobic Exercise – Induced Changes In Serum Mineral Concentrations. African Journal of Biomedical Rsearch 5:13-17. 22- Juel, C.(2007). Changes in interstitial K+ and pH during exercise: implications for blood flow regulation. Appl Physiol Nutr Metab. Oct;32(5):846-51.
23- Szczesna-Kaczmarek, Anna.(2013). Blood K+ concentration balance after prolonged submaximal exercise - The role of both uptake and excretion processes. Gdansk. 5, ( 4): 233-242.
24- Emenike, Smauel; Ifeanyi ,Obeagu Emmanuel.; Ochei, Kingsley Chinedum; Ogbu, Robert Okechukwu; Chineneye, Agoha Silas.(2014). Effect Of Physical Exercises On Serum Electrolyte. IOSR
Journal of Dental and Medical Sciences (IOSR-JDMS) .13(9): 118-121 .
25- Altarawneh, Muath. (2013). Effects of Salbutamol on Potassium and Exercise Performance. A thesis Master, College of Sport & Exercise Science, Victoria University. 26- Nielsen, OB.; de Paoli, FV.(2007). Regulation of Na+-K+ homeostasis and excitability in contracting muscles: implications for fatigue. Appl Physiol Nutr Metab. 32(5):974-84.
27- Juel, C.(2009). Na+, K+ - ATPase in rat skeletal muscle fiber-specific difference in exercise-induced changes in ion affinity and maximal activity. Am J Physiol Regul Integr Comp Physiol. 296:R125-R137. 28- Sejersted , OM.; Sjogaard, G,(2000).. Dynamics and consequences of potassium shifts in skeletal muscle and heart during exercise. Physiol Rev. 80(4):1411-81. 29- McDonough, AA,; Youn, JH. (2005). Role of muscle in regulating extracellular [K+]. Semin Nephrol. 25(5):335-42. 30- Nielsen, OB.; de Paoli, FV . Regulation of Na+-K+ homeostasis and excitability in contracting muscles: implications for fatigue. Appl Physiol Nutr Metab. 2007 Oct;32(5):974 31- Clausen, T.; Gissel, H. (2005). Role of Na, K pumps in restoring contractility following loss of cell membrane integrity in rat skeletal muscle. Acta Physiol Scand. 183(3): 263-71. 32- Hansen, AK.; Clausen. T. ; Nielsen, OB.(2005). Effects of lactic acid and catecholamines on contractility in fast-twitch muscles exposed to hyperkalemia. Am J Physiol Cell Physiol. Jul;289(1):C104-12. 33- Peng, F.L.; Guo, Y.-J.; Mo, W.-B.; Xu, S.-M. and Liao, H.-P.(2014). Cardioprotective effects mitochondrial ATP-sensitive potassium channel in exercise conditioning. Genet. Mol. Res. 13(3): 7503-7512. 34- Anugweje, KC. and Ayalogu, EO.(2014). Effect of Training on the Lactate Dehydrogenase (LDH) levels of Athletes. Researcher.6(9). 35- Adeva-Andany , M.; López-Ojén b,M, ; Funcasta-Calderón a, R.; Ameneiros-Rodríguez a, E.(2014). Comprehensive review on lactate metabolism in human health C. Mitochondrion 17 .76–100.
40- Nikbakht1, Hojatollah.; Ahmad, Abdi.; and Khosro, Ebrahim.(2014). Heart and plasma LDH and CK in response to intensive treadmill running and aqueous extraction of Red Crataegus pentaegyna in male rats. European Journal of Experimental Biology, 4(1): 369-374. 41- Martínez -Porchas, Marcel.; Luis, Rafael Martínez - Córdova.; & Rogelio, Ramos-Enriquez. (2009). Cortisol and Glucose: Reliable indicators of fish stress? Pan-American Journal of Aquatic Sciences .